QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online June 20, 2006

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

Tuba ruffles cells

Scaffolding proteins, such as Tuba, help to coordinate cellular processes by bringing together proteins that need to interact. Frank Gertler and co-authors now suggest that Tuba helps to assemble the molecular machinery needed for certain cytoskeletal rearrangements dependent on N-WASP (see p. 2715). N-WASP, a member of the Wiskott-Aldrich syndrome protein family that regulates actin nucleation, binds to a C-terminal SH3 domain in Tuba. Other domains in Tuba include a GTPase exchange factor (GEF) domain specific for the Rho-family GTPase Cdc42 (which activates N-WASP), a BAR domain (which senses/induces membrane curvature), and several N-terminal SH3 domains that bind dynamin 1 (a GTPase involved in vesicle trafficking). The authors report that overexpression of a Tuba isoform that lacks dynamin-1-binding domains stimulates membrane ruffling in human melanoma cells by interacting with N-WASP and other actin-regulatory proteins; knocking down this isoform by RNAi inhibits the invasive behaviour of these cells. Given these results, the authors conclude that Tuba coordinates signalling and trafficking pathways with cytoskeletal rearrangements during ruffling and other motility processes.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Tuba stimulates intracellular N-WASP-dependent actin assembly

Eva M. Kovacs, Robert S. Makar, and Frank B. Gertler
JCS 2006 119: 2715-2726. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?