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Figure 2


Fig. 2. Ubiquitylation of mutant NPC proteins. COS cells were transfected with myc/His6-ubiquitin (myc/His6-Ubi) together with Flag-NPC1 constructs that encoded the wild-type (wt) or mutant proteins P691S (A) or {delta}LLNF (B); the arrowhead indicates the top of the separating gel. Cells were cultured in the indicated medium for 6 hours (left panels) or treated with or without MG132 for 6 hours (right panels). In both A and B, anti-Flag immunoprecipitation (IP) products were subjected to immunoblotting (IB) with anti-Flag or anti-myc. All results shown are representative and were reproduced at least twice.





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