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Figure 2


Fig. 2. Effects of GIT1 and ßPIX overexpression. (A) Overexpression of GIT1 and ßPIX does not affect the organization of intermediate filaments. Confocal images were taken of COS7 cells cotransfected for GIT1 and ßPIX expression and immunostained for GIT1 (red) and vimentin (green). (B,C) Overexpression of GIT1 and ßPIX partially affects the recycling of transferrin (Tf). A431 cells cotransfected with GIT1 and ßPIX were incubated for 1 hour at 37°C with 60 µg/ml of Alexa Fluor 488-labelled human Tf (green, B), and chased for 2 hours at 37°C (C). Two different confocal planes along the z-axis are shown in B. (D) Overexpression of ßPIX prevented Tf internalization in A431 cells. Cells were fixed, permeabilized and immunostained with antibodies (red) for ßPIX (C,D) or GIT1 (B). Bars, 20 µm.





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