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Figure 1


Fig. 1. CoCl2 treatment provoked MSC differentiation into neuron-like cells. (A) MSC morphological changes observed after 72 hours of CoCl2 treatment by phase-contrast microscopy (objective 20x) and by fluorescence microscopy (objective 40x) after Rhodamine-labelled phalloidin staining (insets). (B) Real-time RT-PCR analyses of neuronal genes and osteogenic genes after 6 and 24 hours of CoCl2 treatment. Values are represented as fold change compared with control ± s.e.m., n=3, *P<0.05, univariate Student's t-test. (C) CoCl2-treated cells were stained for neuronal markers (nestin, Tuj1, NF200 and MAP2a,b) and nuclei were counterstained with Propidium Iodide (PI, in red). Photographs were obtained with confocal microscopy. Bars, 20 µm. (D) Quantification of nestin-, Tuj1- and MAP2ab-positive cells compared with total cells during CoCl2 treatment. *P<0.05, Student's t-test, CoCl2-treated cells were compared with control cells.





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