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Figure 6


Fig. 6. Electrophysiological properties of control MSCs and CoCl2/Y-27632 co-treated MSCs. Treatment with CoCl2 and Y-27632 induced a change in the currents recorded from MSCs. (A) Control cells showed typical inward-rectifying I-V relationship without any sign of voltage-dependent current at depolarizing potentials (n=3). Treated cells, by contrast, clearly exhibited outward-rectifying current similar to that found in neurons (n=10). (B) The outward-rectifying current encountered in CoCl2/Y-27632 co-treated cells was completely blocked by 10 mM TEA (n=3). Insert shows current traces obtained with a voltage step to +60 mV in the presence and absence of 10 mM TEA. Calibration bars, 250 pA 100 milliseconds.





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