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Figure 3


Fig. 3. Endogenous hyperphosphorylated RPA32 (P-RPA) is found within VICE domains during HSV-1 infection. Vero cells were mock-infected (A-D), or infected with HSV-1 (E-I). Pre-extracted cells were triple labeled with mouse anti-UL29 (green), rabbit anti-P-RPA (red) and rat anti-Hsc70 (blue) to detect the nuclear localization of replication compartments, endogenous P-RPA and VICE domains, respectively. (D,H) Merged images for P-RPA and Hsc70 with purple indicating the colocalization of the two proteins. (I) Merged image of UL29, P-RPA and Hsc70 for the HSV-1-infected cell. 100x magnification with 2x zoom.





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