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Figure 4


Fig. 4. VICE domains are excluded from cellular DSBs during HSV-1 infection. Vero cells were mock infected (A-D), or infected with HSV-1, strain KOS (E-I). Pre-extracted cells were triple labeled with mouse anti-UL29 (red), rabbit anti-{gamma}H2AX (green) and rat anti-Hsc70 (blue) to detect the localization of replication compartments, cellular DSBs and VICE domains, respectively. Images were obtained at 100x magnification with 2x zoom. (J-L) Digital enlargements of an area of the cell shown on the left in panels F-H. Arrows indicate typical VICE domains that are exclusive of {gamma}H2AX staining.





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