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Figure 5


Fig. 5. The staining pattern observed for ATR/ATRIP is dependent on the method used for immunolabeling. Mock-infected (A-F) or HSV-1-infected (G-L) cells were either fixed in 4% PFA to visualize total cellular proteins (Fixed, A-C,G-I) or extracted with 0.5% triton X-100 to visualize chromatin-bound and/or matrix-associated proteins (Pre-extracted, D-F,J-L). Cells were double stained using rabbit anti-ATR (red) and mouse anti-ATRIP (green) antibodies. Yellow color indicates a colocalization of the two proteins in the merged images. 100x magnification with 2x zoom.





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