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Figure 6


Fig. 6. ATR is retained in the nucleolus whereas ATRIP is redistributed to VICE domains during HSV-1 infection. Vero cells were infected with HSV-1 (A-D,F-I) or mock infected (E,J). Pre-extracted cells were triple labeled with mouse (A,E) or rabbit (F,J) anti-UL29 (green), rabbit anti-ATR (B,E; red) or mouse anti-ATRIP (G,J; red) and rat anti-Hsc70 (C,E,H,J; blue) to detect the localization of replication compartments, cellular DSBs and VICE domains, respectively. Lavender/pink color indicates the colocalization of ATR or ATRIP with Hsc70 in the merged images. 100x magnification with 2x zoom.





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