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Figure 1


Fig. 1. Assembly properties of GFP-tagged lamin A constructs. (A) Domain structure of the lamin A protein indicating disease-causing mutations that have been analysed in this study. (B) Western blot analysis of HeLa cell lysates expressing GFP-tagged lamin constructs, probed with anti-GFP and anti-lamin A antibodies. Unprocessed and processed forms of lamins are indicated by arrowheads, lamin A del50 is marked by asterisks, and A and C indicate lamin A and lamin C. Molecular mass markers indicated on the left are: phosphorylase b, 94 kDa; albumin, 67 kDa; and ovalbumin, 43 kDa. (C) Immunofluorescence analysis of GFP-tagged lamin A constructs transiently transfected into HeLa cells and counterstained with DAPI. Ut, untransfected cells; WT, wild-type GFP-lamin A; del50, lamin A del50; numbers represent positions of altered amino acid residues indicated in A. Bar, 10 µm.





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