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Fig. 4. Formation of
-H2AX foci after DNA damage. HeLa cells transfected with the indicated GFP-tagged lamin A constructs (green) were (A) treated with cisplatin for 4 hours or (B) irradiated with UV and allowed to recover for 0.5 hours, fixed and stained with antibody to
-H2AX (red) and counterstained with DAPI. Bar, 10 µm. (C,D) Quantitative analysis of
-H2AX staining with cisplatin-treated and UV-treated HeLa cells, respectively. Percentage of untransfected (Ut) or transfected cells positive (solid bars) or negative (open bars) for
-H2AX foci are plotted.