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Figure 6


Fig. 6. Localisation of ATR and ATM kinases in untreated cells. (A) HeLa cells transfected with a FLAG-ATR construct (ATR) or cotransfected with the indicated GFP-tagged lamin A constructs (green) were fixed and stained with anti-FLAG antibody (red), and counterstained with DAPI. Arrows indicate cells showing exclusive cytoplasmic staining of ATR. (B) As in A, except that a FLAG-ATM construct (ATM) was used. Bar, 10 µm. (C) Quantitative analysis of transfected cells expressing ATR, including nuclear and/or cytoplasmic staining (open bars), or ATM (solid bars), both normalised to wild type as 100%.





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