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Figure 4


Fig. 4. mTuba co-localizes with N-WASP, WIP and Toca-1, but not Evl at puncta. (A) B16 cells were co-transfected with GFP-mTuba and mRFP-Evl. Evl localized in discrete bands at the leading edge, distinct from puncta, in mTuba-stimulated dorsal ruffles. (B) B16 cells transfected with GFP-mTuba were cultured on glass coverslips, fixed and stained for N-WASP. Arrowheads indicate robust mTuba and N-WASP co-localization at puncta. (C,D) B16 cells were co-transfected with Cherry-mTuba and either (C) GFP-WIP or (D) GFP-Toca-1. Merged images depict co-localization of mTuba with WIP and Toca-1. Bars: A,C, 3 µm; D, 5 µm; B, 15 µm.





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