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Figure 5


Fig. 5. N-WASP activity is essential for mTuba ruffling. (A) B16 cells were transfected with GFP-mTuba and treated with 10 µM wiskostatin. Images depict mTuba puncta following addition of drug. Wiskostatin abolishes dorsal ruffling and disperses mTuba puncta. (B) Graphical representation of the time-lapse movie from A. Images were thresholded to include the puncta, and the number of thresholded pixels were plotted as a function of time. The intensity of mTuba puncta is reduced by 50% after five minutes treatment and has completely dispersed 8 minutes after addition of the drug (n=3). Bar, 10 µm.





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