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Figure 4


Fig. 4. ROS-mediated signaling is independent of Smad2 phosphorylation. (A) Time-course analysis of TGFß1-initiated phosphorylation of Smad2. Subconfluent HDFs were untreated (C) or treated with 10 ng/ml rTGFß1 CMHDF for the indicated times. The cell lysates were subjected to western blot analysis for phospho-Smad2. The densitometric data (in arbitrary units, a.u.) represent means ± s.e.m. of three independent experiments. (B) Subconfluent HDFs were preincubated with the antioxidants as described (see Fig. 2A, Fig. 3A) before addition of 10 ng/ml rTGFß1 for a further 10 minutes. The cell lysates were subjected to western blotting analysis for phospho-Smad (P-Smad2) and total Smad2/3. Total Smad2/3 was also used as a loading control. Data shown are representative of three independent experiments.





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