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Fig. 2. Absence of endogenous IGF-I reduces P-Akt levels in the OB in vivo. (A) OBs from E16.5-E18.5 Igf-I+/+ and Igf-I/ mouse embryos from the same litters were homogenised and the proteins were analysed by immunoblotting using specific antibodies against P-AktSer473 and Akt. (B) The optical density of the specific bands in immunoblotted membranes was measured by densitometry using Quantity One software. The levels of P-Akt were normalised to the levels of Akt and expressed in arbitrary units. (*P<0.05; Igf-I+/+, n=8; Igf-I/, n=9.)