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Figure 1


Fig. 1. Rho GTPase expression in BMMs derived from Wt, Rac2–/– and Rac1/2–/– mice. (A) To determine the expression profile of Rac isoforms in BMMs, RT-PCR was performed with isoform-specific primers to Rac1, Rac2 and Rac3 on cDNA derived from Wt, Rac2–/– and Rac1/2–/– BMM mRNA. PCR was carried out for 30 cycles. (B) As a positive control for mRNA extraction and cDNA synthesis, RT-PCR was performed with primers to CDK4 on BMM-derived cDNA. PCR was carried out for 30 cycles.





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