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Fig. 3. Rac2^–/– and Rac1/2^–/– macrophages have altered morphology. Wt, Rac2^–/– and Rac1/2^–/– BMMs in growth medium were fixed and stained for F-actin and paxillin or ß-tubulin. (A) The spread area of the basal surface of BMM was measured (see Materials and Methods). (B) The elongation ratio is the ratio of the cell length (longest chord through the cell) to the width (longest axis perpendicular to the longest chord). (C) Confocal images illustrating the stellate, elongated and migratory morphological classification groups. BMMs were stained for F-actin (red) and ß-tubulin (green). (D) Quantification of BMM morphology. BMM shape was defined according to the three classification groups shown in C. Results are shown as mean ± s.e.m. of 150 cells from each population over three separate experiments. *P<0.05 vs Wt BMMs (Student's t-test).

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