JCS02999 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Cells depleted of either Lamin A/C, p150^Glued, CLIP-170 or EB1 (as indicated) were subsequently labelled with Alexa Flour 568-transferrin for 10 minutes followed by imaging at 3 frames per second for 1 minute (see Fig. 6A). No difference is observed in the number, speed or directionality of mobile structures (see text and Fig. 7A). Bars, 5μm. Movies should be played back using QuickTime™ software (www.apple.com/quicktime) with a monitor resolution of 10243768. Please note that all movies have been compressed.

• Movie 2 -

  Movie 2. Cells depleted of either Lamin A/C, p150^Glued, CLIP-170 or EB1 (as indicated) were subsequently labelled with LysoTracker for 5 minutes followed by imaging at 2 frames per second for 1 minute (see Fig. 6C). No difference is observed in the number, speed or directionality of mobile structures. Bars, 10 μm.

• Movie 3 -

  Movie 3. Transport of tsO45-G-YFP from the ER to Golgi was monitored by time-lapse microscopy at 1 frame per second (see Fig. 7A). Cells were shifted to 32°C for 5 minutes before commencement of imaging. Bar, 10 μm.

• Movie 4 -

  Movie 4. Enlargements (23) of the boxed regions (10310 μm) in Movie 3 (Figure 7A) show the presence of VTCs (arrows) that track to the Golgi (see Fig. 7B).

• Movie 5 -

  Movie 5. Cells expressing an intermediate level of GFP-p150^Glued were imaged at 1 frame per second. Boxed region is enlarged 33 in the top right corner and plays back at exactly the same frame rate (see Fig. 8B). Bar, 5 μm.

• Movie 6 -

  Movie 6. Cells expressing a low level of GFP-p150^Glued were imaged at 1 frame per second. Top panel shows the original time sequence, the lower panel shows objects tracking with the trajectories of three dynamic objects overlaid in colour (yellow, cyan and magenta) (see Fig. 8C). Bar, 10 μm.