spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Quantification of plus-end p150Glued. Example quantification of plus-end labelling with anti-p150Glued. (A) Localization of p150Glued at MT plus-ends was determined by immunofluorescence and quantified by analysis of fluorescence intensity of tubulin and p150Glued along a line as shown in the enlargement. Box, 4 µm square. (B) Localization of p150Glued in siRNA transfected cells as indicated. (C) Overlays of the images from B with the corresponding tubulin localization. All panels 42x35 µm in B and C. (D) Graphical representation of fluorescence intensity at plus-ends, calculated as in (A). (E) Quantification of >150 plus-ends for each siRNA transfection (at least ten plus-ends from five cells from three coverslips taken from two independent experiments). The histogram shows that average (mean) intensity values (arbitrary units) with the individual points showing the scatter of the data (notice the close clustering of data points close to the x-axis in EB1 and CLIP-170-depleted cells).





Right arrow Return to article