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Figure 7


Fig. 7. ER-to-Golgi transport is unperturbed in cells depleted of either lamin A/C, p150Glued, CLIP-170 or EB1. (A) tsO45G-YFP transport from the ER to Golgi was monitored by time-lapse microscopy. Cells were shifted to 32°C for 5 minutes before imaging. Bar, 10 µm. See supplementary material Movie 3. (B) Enlargements (2x) of the boxed regions (10x10 µm) in C show the presence of VTCs (arrows) that track to the Golgi (see supplementary material Movie 4). (C) Individual tsO45-G-YFP-labelled VTCs were tracked and grouped into bins of speeds of 0.2 µm s–1. The number of objects in each bin were plotted for experiments in which lamin A/C (x), p150Glued (bullet), EB1 ({triangleup}) or CLIP-170 ({diamondsuit}) had been depleted.





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