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Fig. 1. chs2+ overexpression affects the distribution of some CAR proteins. (A) WT and chs2+-overexpressing cells carrying the Cdc15-GFP protein showing cell wall and nuclear staining (left panels; Cf: Calcofluor) or GFP fluorescence (right panels). The asterisk marks the asymmetric distribution of the Cdc15-GFP ring in a cell. The arrow points to a cell in which the asymmetric Cdc15-GFP ring displays abnormal strong fluorescence. (B) The same analysis as in A was performed in cells carrying Cdc4-GFP. The asterisk indicates a WT cell to show that Cdc4p cannot be observed after the septum has been completed. The arrowhead points to a cell with two Cdc4-GFP rings close together. The diamond marks a cell with a double septum in which the Cdc4-GFP ring did not disassemble after septation. (C) Cells carrying Cdc4-GFP were imaged by confocal microscopy. The same cells are shown from different views. The arrow points to the midzone of a cell in which there are two Cdc4-GFP rings, slightly displaced, that are at different stages of contraction. The asterisks indicates a cell with two rings apparently tilted with respect to each other. Bar, 10µm.
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