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Figure 5


Fig. 5. Chs2p interacts physically with Myo3p and is required for its proper distribution at the ring. Cell extracts from strains carrying the Chs2-HA and/or Myo3-GFP (A and B) and/or the Myo2-GFP (C) fusion proteins were analysed by western blot using the anti-HA ({alpha}-HA) or anti-GFP ({alpha}-GFP) antibodies before (Extracts) or after (IP) immunoprecipitation with the indicated antibody. (D) Distribution of Myo3-GFP in WT or chs2{Delta} cells; the left panels show the transmitted images of the cells and the central panels show a 2D average projection of the series (overlaid to the transmitted images). The graphs show the intensity of relative fluorescence (arbitrary units) plotted against the distance along a line drawn through the cell midzone. Asterisks mark the dots with the strongest relative fluorescence values at both sides of the ring. Myo3-GFP rings are shown in the insets as a 3D reconstruction of the maximum projection of the same z-series.





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