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Figure 2


Fig. 2. Visualization of EB1-GFP reveals the approach of MT plus-end tips toward the wound site. A PtK2 cell expressing EB1-GFP was wounded in 1.8 mM Ca2+ Ringer's solution. The asterisk indicates the site of the cell membrane disruption. A slight increase in the number of bright EB1-GFP `comets' was observed throughout the cell. Furthermore, numerous EB1 comets appeared around the wound site within 30 seconds of membrane disruption (arrowheads). Some comets approached the wound site (arrows). Movies are available in supplementary material (Movies 1 and 2).





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