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Fig. 4. Recruitment of EB1 to the MTs upon cell membrane disruption is dependent on Ca2+. A PtK2 cell expressing EB1-GFP was wounded in 0.4 mM-Ca2+ Ringer's solution. The asterisk indicates the site of cell membrane disruption. A slight increase in the number of bright EB1-GFP comets was observed throughout the cell, however there was no obvious increase in the number of EB1-comets and directional movement of the comets around the wound site in this condition. A movie is available in supplementary material (Movie 3).