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Figure 5


Fig. 5. Disassembly and reassembly of MTs upon cell membrane disruption are independent of PKC activity. A PtK2 cell expressing EB1-GFP was wounded in 1.8 mM Ca2+ Ringer's solution containing 1 µM Gö-6976. The asterisk indicates the site of cell membrane disruption. Numerous EB1-GFP comets appeared within 30 seconds of membrane disruption (arrowheads). Some comets approached the wound site (arrows). A movie is available in supplementary material (Movie 4). A slight increase in the number of bright EB1-GFP comets was also observed throughout the cell.





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