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Figure 6


Fig. 6. Cell membrane disruption stimulates the delivery of NBD lipids toward the wound site along MTs in a PKC- and temperature-dependent manner. PtK2 cells were incubated with 5 µM NBD C6-ceramide/BSA for 30 minutes at 4°C, washed, warmed to 37°C for 30 minutes and wounded with a glass needle. Arrows indicate the sites of cell membrane disruption. Left panel, before wounding; right panel, 5 minutes after wounding. (A) control; (B) 1 µM Gö-6976; (C) 1 µg/ml nocodazole; (D) 20°C. Images are inverted for easier visualization.





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