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Figure 7


Fig. 7. Nocodazole treatment blocked membrane resealing at the second wound. (A) Cells were loaded with 1 µM calcein-AM for 1 hour in the presence or absence of 1 µg/ml nocodazole and wounded twice (arrows). Membrane disruption is indicated by the loss of calcein fluorescence. Bars mark the completion times of membrane resealing. Units of fluorescent intensity were normalized to 100% before the initial wounding. (B) Comparison of membrane resealing rates at the initial and second wounds. The resealing rates were defined as the inverse of the resealing time in seconds. For cells that failed to reseal, the rate was defined as zero. Values are the means ± s.e.m.





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