spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. hTERT is downregulated during NB4-LR1 differentiation. Differentiation (+) of NB4-LR1 cells was obtained by a 72-hour treatment with the combination of ATRA (1 µM) and 8-CPT-cAMP (200 µM). RNA and protein extracts were prepared as described in the Materials and Methods. hTERT mRNA expression was quantified by fluorescence real-time RT-PCR using the LightCycler® technology and the LightCycler TeloTAGGG hTERT Kit from Roche Diagnostics (Meylan, France). The hTERT level was normalized to the expression of the housekeeping gene porphobilinogen deaminase (PBGD) and expressed as a percentage of that detected in the untreated cells. Western blot analyses were performed using RCK-hTERT and NCL-hTERT antibodies. Positions of the molecular weight markers are indicated on the left. Note that the low molecular weight band on NCL-hTERT blot was not reproducibly detected.





Right arrow Return to article