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Fig. 6. Identification of nucleolin and alpha actinin in NB4-LR1 cells by two dimensional electrophoresis and immunoblotting. NB4-LR1 proteins separated by isoelectric focusing in a pH 3-10 Bio-Rad IPG gel in the first dimension and by electrophoresis in an SDS 10% polyacrylamide gel in the second dimension. The proteins were blotted onto PVDF membranes and revealed using the indicated antibodies. Positions of the molecular weight marker are indicated on the left. IP, isoelectric point.