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Figure 7


Fig. 7. Immunofluorescence and confocal microscopy. (A) hTERT was detected in NB4-LR1 cells using RCK-hTERT (red; Alexa Fluor 594-conjugated anti-rabbit antibody). Nucleolin was detected either by MBL-nucleolin (green; Alexa Fluor 488-conjugated anti-mouse antibody) or by SC-nucleolin (red; Alexa Fluor 594-conjugated anti-rabbit antibody). NCL-hTERT antibody detected a protein (green; Alexa Fluor 488-conjugated anti-mouse antibody) that colocalized with nucleolin. Nuclei are stained in blue with DAPI. Panels f and l show the superimposition of RCK-hTERT, NCL-hTERT, DAPI signals and SC-nucleolin, NCL-hTERT and DAPI signals, respectively. (B) Higher magnification of two NB4-LR1 cells stained using either RCK-hTERT (b) or NCL-hTERT (d). Panels c, e and f are superimposed images of DAPI and RCK-hTERT or NCL-hTERT signals and RCK-hTERT and NCL-hTERT signals, respectively. (C) Staining of GM847 cells using either RCK-hTERT (red; Alexa Fluor 594-conjugated anti-rabbit antibody) or NCL-hTERT (green; Alexa Fluor 488-conjugated anti-mouse antibody) antibodies. Nuclei are stained blue with DAPI. Bars, 10 µm.





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