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Fig. 1. Stimulation of Kir3.2 channels by co-expressed wild-type and tagged Gß₁ and G₂ subunits in Xenopus oocytes. (A) K⁺ currents were recorded 1-3 days after injection of cRNAs for the indicated proteins. Data for each sample represent the mean ± s.e.m. for at least five oocytes. The dashed line represents basal Kir3.2 current levels in the absence of co-expressed Gß and G subunits. Note the lack of potentiation when Gß or G constructs were expressed in the absence of their cognate partners. Significant increases in current were determined by an unpaired t-test (*P<0.05 compared with levels in the control), and the data is representative of at least three independent experiments. (B) Agonist-activated currents in oocytes co-expressing ß₂AR. Arrows denote application and washout of 1 µM isoproterenol in KD-98 solution (see Materials and Methods). Oocytes were maintained at –80mV (a potential at which significant inward current can be measured). Data are representative of at least four separate oocytes for each experiment. (C) Data from B normalized to maximal current level to compare activation and deactivation kinetics.

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