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Figure 7


Fig. 7. BRET between tagged effectors and G{alpha} subunits is agonist-independent. HEK 293 cells were transfected to express the indicated proteins and were stimulated or not with 1 µM isoproterenol. (A) BRET was observed in cells co-expressing AC-RLuc and G{alpha}s-GFP though it was not affected by agonist stimulation, and was comparatively less than BRET between AC-RLuc and GFP-G{gamma}2. (B) BRET was also observed in cells stably expressing the ß2AR and co-expressing G{alpha}i-RLuc, Gß1, G{gamma}2 and Kir3.1-GFP. BRET was not affected by agonist regardless of whether or not Kir3.4 was co-expressed. The data represent the mean ± s.d. for at least three experiments.





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