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Figure 6


Fig. 6. Loss of focal adhesions by pharmacological inhibition of PKC{alpha} activity can be overcome by activation of RhoA by LPA. (A) REF cells were plated on FN-coated coverslips and allowed to adhere. At 1.5 hours following seeding, DMSO or Gö6976 (0.5 µM) was added. 30 minutes later some of the cells that had received Gö6976 were either left unstimulated or treated with LPA (400 ng/ml) for 30 minutes (Gö6976 +LPA). Cells were fixed, permeabilized and stained for F-actin and vinculin. Bar, 50 µm. (B) REF cells were transfected with plasmids encoding wt or dnPKD-GFP fusion proteins. 24 hours after transfection cells were either kept in normal growth conditions (+FCS) or serum-starved for 16 hours (–FCS). Cells were fixed and stained for F-actin. Bar, 50 µm.





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