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Figure 1


Fig. 1. Cofilin severing is activated in the leading edge and its severing activity peaks at 60 seconds after stimulation with EGF. (A) Severing activity in cell lysates prepared at the times shown after addition of EGF was measured in an F-actin-severing assay. (Error bars indicate the standard error of the mean (s.e.m.) (B) Pre-incubation of cell lysates with an antibody that blocks cofilin-severing activity abolished the severing-activity peak at 60 seconds after stimulation with EGF (c). However, pre-incubation of nonspecific IgG (d) had no effect on the severing activity peak at 60 seconds after stimulation with EGF, demonstrating that cofilin is the severing protein activated in the cell lysates. Severing activity in the same experiment at 0 seconds (a) and 60 seconds (b) after stimulation with EGF is shown for comparison. (Error bars indicate the s.e.m.) (C) Cofilin-severing acitivity in cell lysates prepared with or without Na3VO4 at the times shown after addition of EGF was measured in an F-actin-severing assay. (D) Changes in the amounts of phosphorylated cofilin with or without the addition of Na3VO4 and NaF.





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