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Figure 2


Fig. 2. The majority of cofilin is dephosphorylated in resting cells. (A) Changes in the percentage of p-cofilin in MTLn3 cell lysates at 0 and 60 seconds after stimulation with EGF were analyzed by isoelectric focusing gel electrophoresis (IEF). (B) Time course of p-cofilin and p-LIMK levels in cell lysates after EGF stimulation analyzed by western blotting with anti-p-cofilin and anti-p-LIMK antibodies (a). Change of p-cofilin levels in cell lysates at resting state and 30 minutes after EGF stimulation analyzed by western blotting with anti-p-cofilin antibodies (b). (C) Western blot quantification of the change in p-cofilin and p-LIMK after EGF stimulation standardized to ß-actin. (Error bars indicate the s.e.m. based on five independent experiments. The analysis of the representative image shown in Fig. 2B falls within the error bars in Fig. 2C.)





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