spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Dephosphorylated cofilin, but not p-cofilin or LIMK1, is associated with the actin cytoskeleton. (A) Representative immunofluorescence images of cofilin and p-cofilin staining in fixed MTLn3 cells compared with Triton X-100-insoluble cytoskeletons. Cofilin is observed throughout the cytoplasm and in the leading-edge compartment in both non-extracted and Triton X-100-extracted cells. However, p-cofilin is not retained in cells extracted with Triton X-100, indicating that it is not bound to the cytoskeleton. (Bar, 10 µm.) (B) Western blot analysis of the retention of cofilin, p-cofilin and LIMK1 in the Triton X-100-insoluble cytoskeleton of MTLn3 cells at 0 and 60 seconds following stimulation with EGF. Western blot was standardized to total protein.





Right arrow Return to article