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Fig. 5. Distribution of LIMK1 and phosphorylated-cofilin. (A) Representative images of LIMK1, p-LIMK1 and p-cofilin immunolocalization in MTLn3 cells fixed at 0 seconds and 60 seconds following stimulation with EGF. The two localization patterns do not significantly overlap. (Bar, 10 µm.) (B) Analysis of LIMK1 (red) and p-cofilin (green) amount from the membrane to inside of the cell 60 seconds after stimulation with EGF, showing the lack of co-enrichment in the leading edge (shaded). (Error bars indicate the s.e.m.) (C) Pearson's correlation coefficient analysis of the overlap of LIMK1 and p-cofilin channels. Both proteins show a low level of correlation throughout and particularly in the leading edge compartment (shaded) at both before (
) and 60 seconds after (
) EGF stimulation. (Error bars indicate the s.e.m.) (D) Pearson's correlation coefficient analysis of the overlap of p-LIMK (red) and p-cofilin (green) channels. Both proteins show a low level of correlation in the leading edge compartment both before and after stimulation. (Error bars indicate the s.e.m.)
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