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Fig. 9. PLC activation is not required for the increase in p-cofilin levels following EGF. (A) MTLn3 cells were preincubated with the PLC inhibitor U-73312 or the inactive control U-73343 for 10 minutes prior to stimulation with EGF. Cells were lysed at 60 seconds following stimulation with EGF and the amount of p-cofilin measured in western blots. (B) Quantification of the p-cofilin level of western blots. The error bars indicate the s.e.m. (n=2 separate experiments).