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Figure 1


Fig. 1. Localisation of the SIN proteins during meiosis. Strains of opposite mating types expressing chromosomally GFP-tagged alleles of Sid4p (A), Cdc11p (B), Spg1p (C), Cdc16p (D), Cdc7p (E), Sid1p (F), Mob1p (G) or Sid2p (H) were mated on EMM-NH4Cl plates at 25°C for 20 hours. Cells were then resuspended in EMM-NH4Cl containing DAPI and visualised under a fluorescence microscope. The columns show cells in the horsetail stage, binucleate cells and tetranucleate cells. In cases where a DAPI image is shown, the DAPI is on the right, the GFP-fluorescence on the left. Bar, 10 µm.





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