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Figure 3


Fig. 3. cdc11-136 is defective in spore formation. (A) Nitrogen-starved pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells were shifted to 34°C for the indicated time and then shifted back to 25°C. Cells were fixed and the percentage of asci that contained spores was determined by phase-contrast microscopy. (B) Kinetics of meiosis in pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells. Starved cells were induced to sporulate at 33°C, and a portion of the culture was fixed at the indicated time and stained with DAPI. Meiotic cells were classified according to the number of nuclei per cell. {diamondsuit}, mononucleate cells; {blacksquare}, binucleate cells; {blacktriangleup}, tetranucleate cells.





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