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Fig. 4. cdc11-136 diploid cells progress normally through the horsetail and the first meiotic division. (A) FACS analysis of diploid pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells growing vegetatively (M), starved in EMM-NH₄Cl during 16 hrs at 25°C (M-N) and induced to sporulate at 33°C for 7 hours (+ NH₄) were analysed for their DNA content as described in materials and methods. (B) Microscopy of living pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing the chromosomally GFP-tagged histone hht2 allele. In the left panel, two images of the same cell are shown, taken 5 minutes apart. (C,D) Observation of living pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing GFP-Atb2p under the control of its own promoter from a plasmid, during the horsetail stage (C) or the first meiotic division (D). Images were recorded at 1-minute intervals. The time after the first images (t=0) is indicated. (E) Live cell microscopy of pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing chromosomally GFP-tagged Pcp1p, to visualise the SPB. The left panel represents the horsetail stage. The position of the SPB is indicated by the white arrow. The right panel shows the first meiotic division. The time (in minutes) after the first image is indicated on each panel. Bars, 10 µm.

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