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Fig. 5. cdc11-136 diploid cells progress normally through the second meiotic division. (A) Observation of living pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing GFP-Atb2p under the control of its own promoter from a plasmid after 6 hours at 34°C. The time in minutes after the first images is indicated on the panels. (B) Observation of living pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing GFP-Hht2p, the nuclear envelope protein Nup107p-GFP, or the kinetochore Nuf2p-GFP. Cells were incubated for 6 hours at 34°C. Bars, 10 µm.