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Figure 6


Fig. 6. Abnormal forespore membrane formation in the cdc11-136 mutant. (A) Observation of living pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing either spo15p-GFP from a plasmid, or chromosomally GFP-tagged alleles of cut12. Cells were incubated for 6 hours at 34°C. (B) Observation of living pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing either spo3p-GFP or psy1p-GFP from a plasmid. Cells were incubated for 6 hours at 34°C. (C) pat1-114/pat1-114 and pat1-114/pat1-114 cdc11-136/cdc11-136 cells expressing Psy1p-GFP were fixed after 6 hours at 34°C as described in Materials and Methods. Forespore membranes and chromatin were examined by using anti-GFP antibody (green) and DAPI (blue), respectively. Bar, 10 µm.





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