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Fig. 8. Ectopic activation of the SIN leads to septation during meiosis. (A) pat1-114/pat1-114 diploid cells carrying a plasmid expressing Spg1 under the control of the nmt1 promoter were grown in minimal medium lacking thiamine for 4 hours at 25°C and then starved in EMM-NH4Cl for 16 hrs at 25°C. Meiosis was induced by incubation at 34°C for 6 hours, when the cells were fixed and stained with DAPI and Calcofluor. The control was cells carrying an empty nmt1 expression vector. (B) Cells were treated as in A, but samples were taken at different time points after induction of meiosis. The septation index in the total population was determined and subdivided into categories of cells containing one, two or four nuclei. (C) Cells were treated as in A. The septation index among the cells containing fournuclei was determined and subdivided into the following three categories: (1) asci containing two nuclei on each side of the division septum (septation occurred at or after MI); (2) asci containing four nuclei on the same side of the division septum (septation occurred before or after MI); (3) multiseptated asci. Bars, 10 µm.
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