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Figure 2


Fig. 2. Atg9 sorting from mitochondria is impaired in sec12 and sed5 cells. (A,B,C) The sec12 atg1{Delta} Atg9-YFP (FRY237) or sed5 atg1{Delta} (FRY303) strains transformed with the vector carrying the atg1ts allele were grown at permissive temperature (24°C) (A; C, left panels) and then shifted to 37°C (B; C, right panels) for 1 hour. Cells were photographed before and after the temperature change; the cells in C were first subjected to mild fixation. (A,B) To visualize mitochondria, MitoFluor Red was added to the culture medium 30 minutes before taking the pictures. The YFP signal is green to facilitate comparison with MitoFluor Red. Control strains were atg1{Delta} Atg9-YFP (FRY138) carrying the plasmid expressing Atg1ts and sec12 Atg9-YFP (FRY154). Arrows indicate colocalization of Atg9-YFP and MitoFluor Red. Arrowheads indicate regions where Atg9-YFP is in close proximity to MitoFluor Red. DIC, differential-interference-contrast images.





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