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Figure 5


Fig. 5. Alteration of subcellular structures in the vps52{Delta} mutant. (A) Mitochondrial morphology is severely compromised in the absence of Vps52. The vps52{Delta} (PSY113) strain was grown in rich medium to an early log-phase and mitochondria labeled by adding MitoFluor Red prior to imaging. (B) Actin cables are aberrantly organized in the vps52{Delta} mutant. Cells grown as in panel A were fixed, permeabilized and incubated with Texas Red-phalloidin (TR-phalloidin) as described in Materials and Methods before collecting fluorescence images. DIC, differential-interference-contrast images.





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