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Figure 6


Fig. 6. Atg9 sorting from mitochondria is blocked in the vps52{Delta} strain in rich medium but not under starvation conditions. (A,B) The vps52{Delta} Atg9-YFP (FRY238) and vps52{Delta} atg1{Delta} Atg9-YFP (FRY192) strains were grown in rich medium (A) or starved in SD-N for 3 hours (B) and then photographed. Mitochondria were labeled by adding MitoFluor Red 30 minutes prior to imaging. Arrows indicate sites of colocalization between Atg9-YFP and MitoFluor Red. Arrowheads indicate regions where Atg9-YFP is in close proximity to MitoFluor Red. DIC, differential-interference-contrast images.





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