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Figure 5


Fig. 5. Effect of short interfering RNAs on tomosyn-1 expression. (A) COS cells were transiently co-transfected with GFP-tagged tomosyn-1 and with empty pSUPER vector (control) or with vectors directing the sequence of two different short interfering RNAs (siRNA-i and siRNA-a). After 3 days the expression level of tomosyn-1 and GFP-tomosyn-1 was analysed by western blotting with a polyclonal antibody against tomosyn-1. The level of tubulin present in the same samples was tested in parallel as a control for protein loading. (B) INS-1E cells were transiently transfected with empty pSUPER vector (control), siRNA-i or siRNA-a. The amount of endogenous tomosyn-1 remaining in the cells after 3 days was assessed by western blotting with antibody against tomosyn-1. Western blotting with antibody against tubulin was performed in parallel as a control for protein loading.





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