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Figure 7


Fig. 7. Capacitance increase after reduction of tomosyn-1 expression. INS-1E cells were transiently transfected with a pSUPER-derived plasmid encoding both GFP and the control siRNA-i (gray) or siRNA-a (black). The transfected cells were identified by EGFP fluorescence and exocytosis was measured by capacitance recordings using the whole-cell configuration of the patch-clamp technique. Exocytosis was triggered by 10x300 ms voltage-clamp depolarizations from –70 mV to 0 mV. **P<0.01, significantly different (unpaired t-test).





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